RealStar Filovirus Screen RT-PCR Kit /96

General description:
The RealStar Filovirus Screen RT-PCR Kit 1.0 is an in vitro diagnostic test, based on real-time PCR technology, for the qualitative detection and differentiation of Ebola- and Marburgvirus specific RNA in human EDTA plasma.

Technical specifications:
Sensitivity: 95%
Specificity: Analytical specificity of the RealStar® Filovirus Screen RT-PCR Kit 1.0 was evaluated by testing a panel of genomic RNA/DNA extracted from different pathogens that are related to Marburg- and Ebolavirus and/or can cause similar symptoms.
Sample type: Plasma, Whole blood
Sample Volume: 140µl to 200µl (depending on the extraction method chosen)
Reading time: extraction process 1h, set up and PCR run: 2,5h for 96well plate (96 samples)

Manufacturer product reference: Altona Diagnostics GmbH, 441013

Items supplied with:
·8 x Master A, 60 μl/Vial
·8 x Master B, 180 μl/Vial
·1 x Internal Control, 1000 μl/Vial
·1 x Positive Control Target Ebola, 250 μl/Vial
·1 x Positive Control Target Marburg, 250 μl/Vial
·1 x Water (PCR grade), 500 μl/Vial

Items required, but not supplied with:
·Appropriate real-time PCR instrument
·Appropriate nucleic acid extraction system or kit
·Desktop centrifuge with a rotor for 2 ml reaction tubes
·Centrifuge with a rotor for microtiter plates, if using 96 well reaction plates
·Vortex mixer
·Appropriate 96 well reaction plates or reaction tubes with corresponding (optical) closing material
·Pipettes (adjustable)
·Pipette tips with filters (disposable)
·Powder-free gloves (disposable)

Shelf life:
15 months from the date of manufacturing.

Storage and transportation condition:
·Should be shipped on dry ice (-20°C). The components of the kit should arrive frozen. If one or more components are not frozen upon receipt, or if tubes have been compromised during shipment, contact Altona Diagnostics GmbH for assistance.
·Should be stored between -25°C and -15°C upon arrival.
·Repeated thawing and freezing of Master reagents (more than twice) should be avoided, as this might affect the performance of the assay. The reagents should be frozen in aliquots, if they are to be used intermittently.
·Storage between +2°C and +8°C should not exceed a period of two hours.
·Protect Master A and Master B from light.

MSDS:
Dry-ice shipment; UN 1845, class 9, carbon dioxide, solid, / Safety file: The Kits do not contain more than 1% of a component classifies as hazardous and do not contain more than 0.1% of a component classified as carcinogenic.