STANDARD M nCoV RealTimeDetectionKit/96T

General description:
STANDARD M nCoV Real-Time Detection kit is used for identification and detection of novel
coronavirus (2019-nCoV) ORF1ab (RdRp) gene and E gene in human nasopharyngeal swab,
oropharyngeal swab, and sputum specimens using reverse transcription (RT) real-time
PCR.

Technical specifications:
Principle: TaqMan probe real-time fluorescent PCR technology
Test format: RT-PCR Instruments
Kit size: 96 Tests
Specimen type: Nasopharyngeal swab, Oropharyngeal swab, Sputum specimens
Specimen volume: 10µl
Time to result: 120 min
Clinical sensitivity: 100%
Clinical specificity: 100%

Manufacturer product reference:
SD Biosensor, M-NCOV-01

Items supplied with:
2 vials x 2019-nCoV Reaction Solution (750µL)
1 vial x RTase Mix (630µL)
1 vial x 2019-nCoV Positive control (600µL)
1 vial x Negative control (600µL)
1 vial x Internal control A (525µL)
1 vial x ROX (55µL)
1x IFU

Items required, but not supplied:
·Instruments:
- LightCycler 480 (Roche).
- CFX96™ Dx System (Bio-Rad),
- Applied Biosystems 7500 Real-Time PCR Instrument System (Thermo Fisher Scientific).
- Applied Biosystems 7500 Fast Real-Time PCR Instrument System (Thermo Fisher Scientific).
·Extraction Kits:
- QIAamp Viral RNA Mini Kit, Qiagen (cat.no. 52904) is recommended by the manufacturer
Other important items:
- Sterilized (DNase, RNase free) micro centrifuge tube (1.5ml).
- Thermal cycler.
- 0.2ml PCR strips, plate (DNase, RNase free) and cap or sealing film for each Real-time PCR equipment.
·General Lab Items:
- Disposable latex gloves
- Sterilized filtered pipette tips.
- Pipettes.
- Vortex mixer.
- Desktop centrifuge.
- Clean bench.
- Flake or snow type ice.
- PPE (Personal Protective Equipment).
- Biohazard waste container.

Shelf life:
12 months from manufacturing date.

Storage & transportation condition:
The kit should be stored in a sealed place at -25°C (-13℉) to -15°C (5℉).
Kit materials are stable until expiration date printed on the outer packaging.
Freeze-thaw times no more than 5 times.

Packaging & labelling:
Primary packaging: All contents of STANDARD M nCoV Real-Time Detection kit of 96T
Secondary packaging: One Kit STANDARD M nCoV Real-Time Detection kit/96T

Weight & volume:
Estimated weight: 0.045 kg
Estimated volume: 0.234 dm³